mouse against dcc icd (Santa Cruz Biotechnology)
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mouse against dcc icd
Mouse Against Dcc Icd, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+against+dcc+icd/DCC+Antibody/pmc11706986-398-26-31
Average 93 stars, based on 66 article reviews
Mouse Against Dcc Icd, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+against+dcc+icd/DCC+Antibody/pmc11706986-398-26-31
Average 93 stars, based on 66 article reviews
mouse against dcc icd - by Bioz Stars,
2026-09
93/100 stars
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1) Product Images from "Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit"
Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit
Journal: Nature Communications
doi: 10.1038/s41467-024-55400-w
Figure Legend Snippet: A Western blot of HEK293T cell lysates transfected with Dcc-pHluorin (Dcc-pH) and JamC-HALO. Immunoprecipitation was performed using GFP or negative control IgG antibodies. Blots were probed for Dcc and HALO-tag, with the yellow arrowhead indicating the expected JamC-HALO band size. B Schematic depicting interactions between Dcc, JamC, and the polarity protein Pard3. Pard3 recruits JamC to the membrane through its PDZ1 domain, which binds the Class 2 PDZ motif at JamC’s C-terminus. JamC interacts with Dcc’s extracellular domain, while Pard3’s PDZ3 the PDZ 3 domain is predicted to interact with a Class 1 PDZ binding motif (X-S/T-X-ϕ COOH ) , , present on Dcc intracellular domain. C , D Airyscan confocal imaging of CGNs nucleofected with Dcc-pHluorin (cyan), JamC-SNAP (yellow), and Halo-Pard3 (magenta). Phluorin and the SNAP dye used here are both pH sensitive highlighting membrane-bound proteins. C Single focal plane showing overlap of Dcc with JamC and Pard3 at the proximal dilation of a CGN (white arrowheads). D Maximum projection of two CGNs forming an adhesion, showing Dcc clustering at the adhesion site before and after Ntn1 addition (200 ng/L). Dcc co-localized with JamC/Pard3 at the adhesion (white arrowhead) and accumulated at the adhesion periphery (hollow arrowhead). Five minutes after the addition of Ntn1 at 200 ng/L, the number of bright Dcc clusters (blue arrowhead) at the membrane surface increased and some newly formed clusters were recruited to the periphery of the JamC/Pard3/Dcc-positive adhesion (white arrowhead). Proximity Labelling Assay (PLA) using Duolink™ fluorescence protocol on fixed dissociated granule neurons plated on laminin and cultured for 24 h, using 2 pairs of primary antibodies: Rabbit against Dcc extracellular domain (ECD) and a Goat against JamC ECD ( E ), and Mouse against Dcc intracellular domain (ICD) and a Rabbit against Pard3 ( F ). Duolink™ staining with no primary, only one or both primaries were compared. Bar graphs represent the ratio of PLA staining intensity (Gray) against Dapi (Cyan) intensity normalized to the negative control without primary antibody (E: n = 4, F: n = 4), replicated 4 (E) and 3 (F) times with similar results. Scale bars: (C, D) 5 µm, (E-F) 10 µm. Error bars represent SEM. See Source Data File.
Techniques Used: Western Blot, Transfection, Immunoprecipitation, Negative Control, Membrane, Binding Assay, Imaging, Fluorescence, Cell Culture, Staining
Related Articles
Western Blot:Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit Article Snippet: Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam 1:200 dil) and Goat against JamC (ECD) (AF1213, R&D systems, 1:50 dil), and Article Title: Antagonistic action of Siah2 and Pard3/JamC to promote germinal zone exit of differentiated cerebellar granule neurons by modulating Ntn1 signaling via Dcc Article Snippet: Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam) and Goat against JamC (ECD) (AF1213, R&D systems), and Transfection:Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit Article Snippet: Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam 1:200 dil) and Goat against JamC (ECD) (AF1213, R&D systems, 1:50 dil), and Article Title: Antagonistic action of Siah2 and Pard3/JamC to promote germinal zone exit of differentiated cerebellar granule neurons by modulating Ntn1 signaling via Dcc Article Snippet: Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam) and Goat against JamC (ECD) (AF1213, R&D systems), and Immunoprecipitation:Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit Article Snippet: Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam 1:200 dil) and Goat against JamC (ECD) (AF1213, R&D systems, 1:50 dil), and Article Title: Antagonistic action of Siah2 and Pard3/JamC to promote germinal zone exit of differentiated cerebellar granule neurons by modulating Ntn1 signaling via Dcc Article Snippet: Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam) and Goat against JamC (ECD) (AF1213, R&D systems), and Negative Control:Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit Article Snippet: Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam 1:200 dil) and Goat against JamC (ECD) (AF1213, R&D systems, 1:50 dil), and Article Title: Antagonistic action of Siah2 and Pard3/JamC to promote germinal zone exit of differentiated cerebellar granule neurons by modulating Ntn1 signaling via Dcc Article Snippet: Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam) and Goat against JamC (ECD) (AF1213, R&D systems), and Membrane:Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit Article Snippet: Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam 1:200 dil) and Goat against JamC (ECD) (AF1213, R&D systems, 1:50 dil), and Article Title: Antagonistic action of Siah2 and Pard3/JamC to promote germinal zone exit of differentiated cerebellar granule neurons by modulating Ntn1 signaling via Dcc Article Snippet: Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam) and Goat against JamC (ECD) (AF1213, R&D systems), and Binding Assay:Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit Article Snippet: Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam 1:200 dil) and Goat against JamC (ECD) (AF1213, R&D systems, 1:50 dil), and Article Title: Antagonistic action of Siah2 and Pard3/JamC to promote germinal zone exit of differentiated cerebellar granule neurons by modulating Ntn1 signaling via Dcc Article Snippet: Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam) and Goat against JamC (ECD) (AF1213, R&D systems), and Imaging:Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit Article Snippet: Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam 1:200 dil) and Goat against JamC (ECD) (AF1213, R&D systems, 1:50 dil), and Article Title: Antagonistic action of Siah2 and Pard3/JamC to promote germinal zone exit of differentiated cerebellar granule neurons by modulating Ntn1 signaling via Dcc Article Snippet: Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam) and Goat against JamC (ECD) (AF1213, R&D systems), and Fluorescence:Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit Article Snippet: Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam 1:200 dil) and Goat against JamC (ECD) (AF1213, R&D systems, 1:50 dil), and Article Title: Antagonistic action of Siah2 and Pard3/JamC to promote germinal zone exit of differentiated cerebellar granule neurons by modulating Ntn1 signaling via Dcc Article Snippet: Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam) and Goat against JamC (ECD) (AF1213, R&D systems), and Cell Culture:Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit Article Snippet: Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam 1:200 dil) and Goat against JamC (ECD) (AF1213, R&D systems, 1:50 dil), and Article Title: Antagonistic action of Siah2 and Pard3/JamC to promote germinal zone exit of differentiated cerebellar granule neurons by modulating Ntn1 signaling via Dcc Article Snippet: Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam) and Goat against JamC (ECD) (AF1213, R&D systems), and Staining:Article Title: Siah2 antagonism of Pard3/JamC modulates Ntn1-Dcc signaling to regulate cerebellar granule neuron germinal zone exit Article Snippet: Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37 °C for 24 h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam 1:200 dil) and Goat against JamC (ECD) (AF1213, R&D systems, 1:50 dil), and Article Title: Antagonistic action of Siah2 and Pard3/JamC to promote germinal zone exit of differentiated cerebellar granule neurons by modulating Ntn1 signaling via Dcc Article Snippet: Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.Proximity labelling assay 28 Proximity labelling assay was carried out using Duolink® system (Sigma-Aldrich), following the manufacturer instructions and recommended controls, on dissociated CGNs plated on laminin and cultured at 37ºC for 24h after fixation 2% PFA/0.5% Glutaraldehyde.. Two pairs of primary antibodies were used: Rabbit against Dcc (ECD) (ab273570, Abcam) and Goat against JamC (ECD) (AF1213, R&D systems), and |